| KU812 와 RBL-2H3 비만세포에서 interleukin-8(IL-8) 발현 및 histamine 유리에 대한 플라보노이드의 효과 |
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구동환, 한상철, 강경진, 강희경, 유은숙 |
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제주대학교 의학전문대학원 약리학교실 |
| Effects of Several Flavonoids on KU812 and RBL-2H3 mast cell line |
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Dong-Hwan Koo, Sang-Chul Han, Gyroung-Jin Kang, Hee-Kyoung Kang, Eun-Sook Yoo |
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Department of Pharmacology, Jeju National University School of Medicine, Jeju, Korea |
Correspondence:
Eun-Sook Yoo, Email: eunsyoo@jejunu.ac.kr
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| Abstract |
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Allergic disease including atopic dermatitis (AD), bronchial asthma and rhinitis are inflammatory responses. AD is a chronic, recurrent inflammatory skin disease that have symptoms such as pruritus. AlIergic responses associate with mast cells which play as the major effector cells in immediate hypersensitivity (type I response) through activation via the high-affinity IgE receptor, Fce RI, although many other functions have recently been discovered for this cell type. Interleukin-8 (IL-8) is a cytokine that produced by various types of cells upon stimulated by antigen, inflammatory stimuli and exerts a variety of functions on mast cells and neutrophils. Release of histamine is a degranulation marker in simulated mast cells. Flavonoids are chemical substance in plants have antl-bacterial, anti-viral and anti-inflammatory effects. We investigated the effects of flavonoids on the expression of IL-8 on KU812 cells using flow cytometry and the inhibition of histamine release on RBL-2H3 cells. Mast cells stimulated with phorbol myristate acetate (PMA) plus calcium ionophore A23187 and treated with flavonoids suppressed IL-8 and histamine. We found that most of flavonoids inhibited the expression of IL-8 on KU812 cells and the histamine release on RBL-2H3 cells. Therefore, these results suggest that flavonoids have connection with expression of IL-8
and inhibition of histamine release in mast cells. |
| Key Words:
KU812; RBL-2H3; Flavonoid; Interleukin-8; Histamine |
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