J Med Life Sci > Volume 9(2); 2012 > Article
Journal of Medicine and Life Science 2012;9(2):97-102.
DOI: https://doi.org/10.22730/jmls.2012.9.2.97    Published online December 18, 2012.
Glucocorticoid 에 의한 성상교세포와 미세신경교세포에서의 유전자 발현상의 변화
최연희, 정성철, 은수용
제주대학교 의학전문대학원 생리학교실
Effects of glucocorticoid treatment on gene expression in astrocytes and microglia
Yan-Ji Cui, Sung-Cherl Jung, Su-Young Eun
Department of Physiology, Jeju National University School Medicine, Jeju, Korea
Correspondence:  Su-Young Eun, Email: syeun@jejunu.ac.kr
Received: 20 November 2012   • Revised: 27 November 2012   • Accepted: 4 December 2012
Abstract
In the present study. we investigated cell type-specific effects of glucocorticoid on cellular function and gene expression in different brain cells. Dexamethasone (DEX, ㎛), a synthetic glucocorticoid, was treated for 24 h in HT-22 neurons, C6 astrocytes and BV-2 microglial cells. DEX Irealment significantly increased cellular mRNA levels of crystallin alpha B in C6 astrocytes and the effects were reversed by glucocorticoid receptor (GR) antagonist RU486 (Mifepristone). Lipopolysaccharide (LPS, 200 ng/ml) -induced NO release, nuclei translocation of NF-κB, degradation of cytosol IκB, and mRNA expression of TNF-alpha in microglia, a well-known phenomenon presenting pro-inflammatory capacity of microglia, was suppressed by DEX treatment. These effects were reversed by RU486 treatment. Our results suggest that glucocorticoid might deteriorate neuronal survival and maintenance of function. Astrocytes and microglia might protect damaged neurons vulnerable to stress hormone. Taken together, we postulate neuron-glia interaction and the specific roles of neurons, astrocytes, and microglia in stress responses and stress-related psychiatric disorders such as depression.
Key Words: Slress; Glucocorticoid; Neurons; Astrocytes; Microglia; Neuron-gIia interaction
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