Effect and mechanism of Docosahexaenoic acid on the proliferation of dermal papilla cells

Ko, Jiyeon; Oh, Il-Joong; Kang, Jung-Il; Choi, Youn Kyung; Yoon, Hoon-Seok; Yoo, Eun-Sook; Ko, Chang-Ik; Ahn, Yong-Seok

doi:2019.16.3.84

J Med Life Sci > Volume 16(3); 2019 > Article

Original Article

Journal of Medicine and Life Science 2019;16(3):84-89.
DOI: https://doi.org/10.22730/jmls.2019.16.3.84 Published online January 16, 2020.

Docosahexaenoic acid의 모유두세포 증식 효능 및 기전

, , , , , , ,

Effect and mechanism of Docosahexaenoic acid on the proliferation of dermal papilla cells

Jiyeon Ko, Il-Joong Oh, Jung-Il Kang, Youn Kyung Choi, Hoon-Seok Yoon, Eun-Sook Yoo, Chang-Ik Ko, Yong-Seok Ahn

Department of Medicine, School of Medicine, Jeju National University
Department of Medicine, School of Medicine
Choung Ryong Fisheries Co. Ltd.

Abstract

Docosahexaenoic acid (DHA), a principal of mackerel-derived fermented fish oil, increases the proliferation of dermal papilla cells (DPCs) via the upregulation of cell cycle-associated proteins such as cyclin D1 and cdc2 p34, and might promote hair-growth. However, the intracellular mechanisms that underlie the action of DHA in the proliferation of DPCs have not been investigated fully. In this study, we addressed the action mechanisms of DHA to trigger the activation of anagen in DPCs. DHA activated β-catenin signaling by the increased phosphorylation at serine 552 and serine 675 as well as the translocation and accumulation of activated β-catenin into the nucleus. In the other hand, DHA inhibited canonical TGF-β/Smad signaling by the decreased phosphorylation of Smad2/3. Taken together, the results indicate that DHA might stimulate anagen signaling via the activation of Wnt/β-catenin pathway, while the inactivation of canonical TGF-β signaling pathway in DPCs.

고등어 발효 어유의 주성분인 docosahexaenoic acid (DHA)은 cyclin D1과 cdc2 p34와 같은 세포 주기 관련 단백질의 발현 증가를 통해 dermal papilla cells (DPCs)의 증식을 증가시키며 모발 성장을 촉진할 수 있다. 그러나 DPCs의 증식에서 DHA의 작용의 기초가 되는 세포내 메커니즘은 충분히 조사되지 않았다. 본 연구에서는 DPCs에서 모낭의 성장기의 활성화를 유발하기 위한 DHA의 작용 메커니즘을 다루었다. DHA는 β-cetenin의 ser552 및 ser675 부위의 인산화 증가와 활성화된 β-cetenin의 핵으로의 이동 및 축적에 의해 β-catenin 신호를 활성화하였다. 한편, DHA는 Smad2/3의 인산화 감소에 의해 canonical TGF-β/Smad 신호를 억제하였다. 결과를 종합해 보면, DHA가 DPCs에서 canonical TGF-β 신호 경로를 비활성화하며 Wnt/β-catenin 경로의 활성화를 통해 모낭의 성장기 신호전달을 자극할 수 있음을 시사한다.

Key Words: Docosahexaenoic, acid;, Dermal, papilla, cells;, Wnt/β-catenin, pathway;